PRODUCTION OF TAXOL BY CLADOSPORIUM SPECIES ISOLATED FROM AIR, OKRA LEAVES AND FRUITS OF GRAPEVINE

Document Type : Novel Research Articles

Abstract

A great attention was paid towards exploiting microorganisms for cheap production of different bioactive compounds including paclitaxel (taxol) as a highly effective and broad spectrum natural anticancer agent. In this study five species of Cladosporium isolated from outdoor air, okra leaves and grapevine fruits in Egypt were tested for their abilities to produce paclitaxel. The fungal species were identified morphologically and by molecular techniques (sequencing ITS region of rDNA). Sequences were deposited in the GenBank as Cladosporium halotolerans AUMC 11387 (GenBank accession No. MN826823), C. limoniformis AUMC 11301(GB: MN826827), C. sinusum AUMC 11340 (GB: MN826919), C. spharospermum AUMC 10865 (GB: MN826828), C. xanthochromaticum AUMC 11366 (GB: MN826822). The fungal strains were grown on potato dextrose broth (PDB) and the paclitaxel was extracted using ethyl acetate. The fungal taxol was detected by thin layer chromatography (TLC) and evaluated quantitatively by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). Results showed that all tested species were able to produce taxol but in variable levels. The best producers were C. halotolerans (5.943ug/L) and C. sphaerospermum (3.074ug/L). The remaining Cladosporium species yielded lower quantities of taxol ranging from 0.410 to 1.844ug/L. The study introduces Cladosporium species as important candidates for taxol production.

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