LIPOLYTIC ACTIVITY OF FUNGI ASSOCIATED WITH SOME OILY-SEEDS AT ASSIUT GOVERNORATE, EGYPT

Abstract

A total of 27 fungal species and one variety related to 9 genera were recovered from sesame (21+1 & 7), peanut (12 & 5) and soybean (19+1 & 6) gathered from different stores at Assiut Governorate. Aspergillus was the most prevalent genus whereas, the most common species were A. niger, and A. flavus. Out of 134 fungal isolates tested for lipase production, only 39 isolates have lipolytic properties and A. niger 49sp and 38sp recovered from soybean seeds were the highest producers giving each 1.07 cm for lipase activity. Using 1% NaCl solution, 1% NaCl + 1% tween 80, and phosphate buffer (pH 7.0) for extracting lipase enzyme from sesame, groundnut and soybean seeds. It was proved the high efficiency of 1% NaCl + 1% tween 80 in the extraction with four concentrations through the three seeds. The current study proved the high efficiency of oilseeds borne fungi for lipase production and the important of using the right extraction solvent. Also, this study is the precedent for further trials, where it is intended to improve the production of the enzyme lipase to be purified and commercialized.

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