EFFECTS OF BRODIFACOUM, ACETYLSALICYLIC ACID AND ITS COMBINATION ON HAEMOSTATIC, HEMATOLOGICAL AND HEPATIC MARKERS OF WILD RAT RATTUS RATTUS

doi:10.21608/aunj.2019.221121

EFFECTS OF BRODIFACOUM, ACETYLSALICYLIC ACID AND ITS COMBINATION ON HAEMOSTATIC, HEMATOLOGICAL AND HEPATIC MARKERS OF WILD RAT RATTUS RATTUS

Document Type : Novel Research Articles

10.21608/aunj.2019.221121

Abstract

Development of resistance in rodents against rodenticides represents a major challenge for rat management strategies in the light of heavy damage and infestation of crop fields. The aim of this study is to examine the effects of brodifacoum (BDF), acetylsalicylic acid (ASA), and a novel formulation of both anticoagulants on haemostatic and hematological parameters, hepatic redox status and histoarchitecture. Forty eight adult female wild rats (Rattus rattus) were randomly and equally divided into three groups, eight rats each. BDF induced a significant increase in bleeding and prothrombine time, lipid peroxidation (LPO) and decrease in factor VII and IX and hepatic nitric oxide (NO) levels and catalase (CAT) activity, and eosinophils, lymphocytes and monocytes count. Rats in the combination group were characterized by a significant increase in clotting and prothrombin time and ionized calcium (Ca⁺²) level together with a significant decrease in factors VII, IX and NO levels, platelet count, mean platelet volume, and hepatic CAT activity. ASA was responsible for a significant increase in bleeding time, factor IX level and hepatic CAT activity, and decrease in hepatic NO level. Adverse changes in hepatic histoarchitecture were observed following each of BDF and ASA exposure. The combination of ASA with BDF further aggravated the histopathological lesions of liver more than BDF alone. It was concluded that ASA induced a joint action with BDF especially regarding the haemostatic markers and hepatocellular features.

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