The Role of Fragmented Adipose Mesenchymal Stem Cells in Vitro Therapy for Human Hepatoma Cell Line Growth

Document Type : Novel Research Articles

Authors

1 Zoology department, faculty of science, Assuit university , Egypt

2 Department of Biology, Faculty of Education, Taiz University, Yemen.

3 Zoology Department, Faculty of Science, Assiut University, Egypt

Abstract

Background: Stem cells (SCs) from adipose tissue are being studied as part of regenerative medicine and used in vitro tissue engineering. Aim: The study goal was to highlight the modifications of fragmented adipose mesenchymal stem cells (AD-MSCs) on human hepatoma cell lines (HepG2 and Huh7) by inducing cell death (either apoptosis or necrosis). Materials and Methods: This study explains the methods of isolating, identifying, and characterization of fragmented frozen AD-MSCs. Also, investigate the phenotype of AD-MSCs culturing cells obtained in Passage 3 using CD105 & CD90 as positive markers and CD45 as a negative marker. Two Hepatoma cell line (HCC) cell lines were cultured in RPMI 1640/10%. The viability and apoptosis of the HCC cell line were evaluated by MTT and AO/EB methods, respectively. Results: Showed that, compared to CD45, the percentages of brown dots in the AD-MSC nucleus and cytoplasm increased for CD105 and CD90. HepG2 and Huh7 cells' growth and apoptosis were affected by the perceived seeding of fragmented AD-MSCs. Also, increasing the fragmented AD-MSC concentration (from 1:15 μl for 24 h to 1:30 μl for 48 h) in vitro decreased HCC cell line viability, in which Huh7 was more susceptible than HepG2 after 24–48 h of incubation time (p < 0.001), as assessed by MTT. Moreover, cell counting by AO/EB assays appeared to initiate cell death, which increased in Huh7 cells compared to HepG2 cells. Conclusion: New evidence suggests that fragmented AD-MSCs could be highly efficient in preventing carcinogenesis by inducing apoptosis in HCC cell lines.

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Assiut University Journal of Multidisciplinary Scientific Research
Volume 1, Issue 1 - Serial Number 1
The 7 th Conference for Young Scientists in Basic and Applied Sciences, May 10-11th, 2022.
2022
Pages 73-94

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