Green Magnesium Oxide Nanoparticles (MgO-NPs) As An Antibacterial Agent Against Multidrug-Resistant Bacteria Causing Tonsillitis

Document Type : Novel Research Articles

Authors

1 Molecular Biology Researches and Studies Institute

2 Professor of Microbiology, Assiut University, Faculty of Science

3 Electronics & Nano Devices Lab, Physics Department, Faculty of Science, South Valley University

4 Botany & Microbiology Department, Faculty of Science, South Valley University

10.21608/aunj.2025.363865.1117

Abstract

In this study, the potential of magnesium oxide nanoparticles (MgO-NPs) as an antibacterial agent against multidrug-resistant bacteria causing tonsillitis was investigated. The MgO-NPs were synthesized using either Neem, Black seeds, or NaOH (MgO-NPs/Neem, MgO-NPs/Black seeds, and MgO-NPs/NaOH). The efficacy of these nanoparticles on the biofilm of three bacteria (Staphylococcus aureus (MRSA), ß-hemolytic Streptococci (BHS) Streptococcus pyogenes, and α-hemolytic Streptococci Streptococcus pneumoniae) was evaluated. The morphology and structure of the MgO-NPs were characterized using various techniques such as X-ray diffraction (XRD), High-Resolution Transmission Electron Microscopy (HRTEM), Fourier Transform Infrared Spectroscopy (FTIR), and UV-vis Spectroscopy. Both HRTEM and XRD revealed the MgO-NPs as irregular spherical-shaped and the crystallite sizes of the MgO samples were determined to be 18.5 nm, 16.4 nm, and 18.2 nm for those prepared from NaOH, Neem extract, and Black seeds extract, respectively. The results demonstrated that MgO-NPs exhibited minimum inhibition concentration (MIC) ranging from 450 µg/ml to 540 µg/ml. Additionally, MgO-NPs showed effective activity against biofilm-forming pathogenic bacteria. These findings suggest that MgO-NPs have significant potential as cost-effective and environmentally friendly antibacterial and anti-biofilm agent for controlling and preventing biofilm-related infections in future clinical treatments.

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